Legionella effectors that promote nonlytic release from protozoa

Publication Type  Journal Article
Year of Publication  2004
Authors  Chen, J.; de Felipe, K. S.; Clarke, M.; Lu, H.; Anderson, O. R.; Segal, G.; Shuman, H. A.
Journal Title  Science
Volume  303
Issue  5662
Pages  1358-1361
Journal Date  Feb 27
ISBN Number  0036-8075
Accession Number  ISI:000189238600048
Key Words  legionnaires-disease; human-monocytes; acanthamoeba-castellanii; pneumophila; cells; phagosome; dictyostelium; system; growth; amebas

Legionella pneumophila, the bacterial agent of legionnaires' disease, replicates intracellularly within a specialized vacuole of mammalian and protozoan host cells. Little is known about the specialized vacuole except that the Icm/Dot type IV secretion system is essential for its formation and maintenance. The Legionella genome database contains two open reading frames encoding polypeptides (LepA and LepB) with predicted coiled-coil regions and weak homology to SNAREs; these are delivered to host cells by an Icm/Dot - dependent mechanism. Analysis of mutant strains suggests that the Lep proteins may enable the Legionella to commandeer a protozoan exocytic pathway for dissemination of the pathogen.


778JNTimes Cited:85Cited References Count:26

URL  <Go to ISI>://000189238600048