Phylogenetic diversity and evolutionary relatedness of alkenone-producing haptophyte algae in lakes: Implications for continental paleotemperature reconstructions

Publication Type  Journal Article
Year of Publication  2010
Authors  Theroux, Susanna; D'Andrea, William J.; Toney, Jaime; Amaral-Zettler, Linda; Huang, Yongsong
Journal Title  EARTH AND PLANETARY SCIENCE LETTERS
Volume  300
Pages  311–320
Journal Date  DEC 1
Abstract  

Alkenones have been found in an increasing number of lakes around the world, making them a promising new tool for continental paleoclimate reconstruction. However, individual lakes may harbor different species of haptophyte algae with different sensitivities to temperature variations, thus presenting a significant challenge to the use of lacustrine alkenones for paleotemperature reconstructions. To explore the extent of lacustrine haptophyte diversity, we conducted the first comprehensive phylogenetic and geochemical survey of lacustrine alke lone producers. We sampled 15 alkenone-containing lake surface sediments from a variety of geographic locales and inferred identities of environmental sequences using 18S ribosomal RNA (rRNA) gene-based phylogenies. For two lakes, BrayaSo in southwest Greenland and Tso Ur on the Tibetan Plateau, we also analyzed both surface and downcore sediments to characterize haptophyte populations through time. In parallel with phylogenetic analyses, we determined the alkenone distributions (including C-37/C-38 ratios, and the presence/absence of C-38 methyl ketones and C-40 compounds) in all the samples. The resulting alkenone profiles from this study do not all align with traditional ``marine'' versus ``coastal/lacustrine'' alkenone profiles. Additionally, our genetic data indicate the presence of multiple haptophyte species from a single lake sediment sample; these distinct haptophyte populations could not be discerned from the alkenone profiles alone. These results show that alkenone profiles are not a reliable way to assess the haptophyte algae in lakes and that DNA fingerprinting is a preferred approach for species identification. Although closely related haptophyte species or subspecies may not warrant different temperature calibrations, our results emphasize the importance of genetic data for inferring haptophyte identities and eventually selecting alkenone temperature calibrations for paleoclimate reconstructions. (C) 2010 Elsevier B.V. All rights reserved.