Anderson, O.R., Lin, G. Gregory-Wodzicki, K.M., and Griffin, K., submitted 11/98 to American Journal of Botany, Effects of elevated CO2 concentrations on leaf fine structure, physiognomy and photosynthesis of some C-3 and C-4 plants grown in Biosphere 2


Plants grown in Biosphere 2 at elevated carbon dioxide concentrations and those grown in nearby greenhouses at ambient (normal) carbon dioxide concentrations were examined for differences in fine structure and leaf morphology. Chloroplasts of C-3 plants (Carica papaya, Clitoria racemosa, Epipremnum pinnatum, Musa acuminata, and Piper auritum) grown in elevated atmospheric carbon dioxide in Biosphere 2 have longer intergrana thylakoids that occupy a greater proportion of the chloroplast volume than the same species grown in greenhouses at ambient CO2 levels (p<<0.01). There were no differences for C-4 plants including grasses (Pennisetum ciliare, and Pennisetum purpurea) and Atriplex cinerea. Among the species studied for leaf morphological analyses (Rhizophora mangle, Laguncularia racemosa, Avicennia germinans, Simmondsia sinensis, Hyptis emoryi, Clitoria racemosa, and Atriplex cinerea) there are no consistent differences in leaf size, shape, or tooth frequency related to CO2 concentration; rather, the data are more consistent with the hypothesis that phenotype and microclimate account for more of the observed variance than carbon dioxide concentrations.