Cryo-archiving of Batrachochytrium dendrobatidis and other chytridiomycetes

Publication Status is "Submitted" Or "In Press: 
LDEO Publication: 
Publication Type: 
Year of Publication: 
2003
Editor: 
Journal Title: 
Diseases of Aquatic Organisms
Journal Date: 
Aug 15
Place Published: 
Tertiary Title: 
Volume: 
56
Issue: 
1
Pages: 
59-64
Section / Start page: 
Publisher: 
ISBN Number: 
0177-5103
ISSN Number: 
Edition: 
Short Title: 
Accession Number: 
ISI:000185983100007
LDEO Publication Number: 
Call Number: 
Abstract: 

Batrachochytrium dendrobatidis is a major pathogen of frogs worldwide. It has been associated with catastrophic declines of frog populations including those in pristine habitats in Queensland, Australia. To facilitate genetic and disease studies of this fungus and related species, it is essential to have a reliable long-term storage method to maintain genetic integrity of isolates. We have adapted well-established techniques used for the long-term storage of tissue-culture cell lines to the preservation of B. dendrobatidis and other chytridiomycetes. This simple method has allowed us to recover these fungi from storage at -80degreesC and in liquid nitrogen over an extended period. With this technique it is now possible to preserve saprobic and parasitic isolates from a variety of environmental and disease situations for comparative genetic and biological studies.

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733CVTimes Cited:8Cited References Count:7

DOI: